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image processing software  (Nikon)


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    Nikon image processing software
    Image Processing Software, supplied by Nikon, used in various techniques. Bioz Stars score: 99/100, based on 39732 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/image+processing+software+nis+elements+microscopy+software/NIS-Elements/10__1002_slash_aff2__85-113-19-26
    Average 99 stars, based on 39732 article reviews
    image processing software - by Bioz Stars, 2026-09
    99/100 stars

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    Related Articles

    Bicinchoninic Acid Protein Assay:

    Article Title: Exercise-induced crosstalk between immune cells and adipocytes in humans: Role of oncostatin-M
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) 1:1000 Cell Signaling Technology #9101; RRID:AB_331646 Phospho-HSL (Ser563) 1:1000 Cell Signaling Technology #4139; RRID:AB_2135495 Phospho-Stat3 (Tyr705) (D3A7) XP 1:1000 Cell Signaling Technology #9145; RRID:AB_2491009 Itgam 1:500 Atlas Antibodies AMAb90911; RRID:AB_2665722 Biological samples Primary human myoblasts Previous works from the group N/A Human in vitro differentiated adipocytes Gao et al., 201759 N/A Human subcutaneous leg fat biopsies This paper N/A Human plasma samples This paper N/A Human subcutaneous abdominal fat biopsies Fabre et al.20 N/A Chemicals, peptides, and recombinant proteins Oncostatin-M Bio-Techne LTD 295-OM-050 GDF15 Bio-Techne LTD 9279-GD-050 SFRP4 Bio-Techne LTD 1827-SF-025/CF MRXA5 Nordic Biosite LS-G26239-10 Isoproterenol Merck I5627 Clenbuterol Merck C5423-10MG Forskolin Merck F6886-10MG IBMX Merck I5879-250MG Insulin Novo Nordisk Actrapid Dexamethasone Merck D4902-1G Rosiglitazone GlaxoSmithKline BRL49653-C apo-transferrin BBI solution T100-5 Recombinant Human IL4 PeProTech 200–04 Recombinant Human IL13 PeProTech 200–13 Recombinant Human IFNg PeProTech 300–02 Phorbol 12-myristate 13-acetate (PMA) Merck/Sigma-Aldrich P1585 Lipopolysaccharides Merck/Sigma-Aldrich L9641 Hoechst 33342 Abcam Ab228551 Lectin Vector Laboratories DL-1048-1 TRIzol Thermo Fisher Scientific 15596018 Triton X-100 Sigma-Aldrich X100-100ML Tween 20 Sigma-Aldrich P1379-1L DAKO Fluorescence mounting media Agilent Technologies S302380-2 Protein Block Agilent Technologies X090930-2 Protease Inhibitor Cocktail CALBIOCHEM 539131–10 ECL reagent GE Healthcare RPN2106 Critical commercial assays TruSeq Stranded Total RNA with Ribo-Zero Gold Illumina 20020598 Rneasy lipid tissue kit Qiagen Cat#74804 Agilent RNA 6000 nano kit Agilent 5067–1511 Qubit dsDNA HS assay kit Invitrogen Q32851 Agilent High Sensitivity DNA chip Agilent 5067–4626 Human Oncostatin M/OSM ELISA Kit Abcam ab215543 (Continued on next page) e1 Cell Reports Medicine 5, 101348, January 16, 2024 .. REAGENT or RESOURCE SOURCE IDENTIFIER EZNA total RNA kit I Omega Bio-tek R6834-01 Pierce BCA Protein Assay Kit ThermoFisher Cat#23227 Multiplex Fluorescent detection reagents v2 ACDBio 323110 Free glycerol Reagent Sigma-Aldrich #F6428 Amplex Ultrared Reagent Invitrogen #A36006 Deposited data Human adipose tissue in response to exercise (RNAseq) This paper GEO: GSE198922 Experimental models: Cell lines Thp1 monocytes cell line ATCC TIB-202 Oligonucleotides TaqMan probe human OSM Applied Biosystems Hs00171165_m1 TaqMan probe human GDF15 Applied Biosystems Hs00171132_m1 TaqMan probe human LIF Applied Biosystems Hs01055668_m1 TaqMan probe human OSMR Applied Biosystems Hs00384276_m1 TaqMan probe human LIFR Applied Biosystems Hs01123581_m1 TaqMan probe human IL6ST Applied Biosystems Hs00174360_m1 TaqMan probe human IL6 Applied Biosystems Hs00174131_m1 TaqMan probe human CXCL2 Applied Biosystems Hs00601975_m1 RNAscopeTM Probe- Hs-OSM ACDBio 456381 RNAscopeTM Probe- Hs-CD68-C2 ACDBio 560591-C2 Software and algorithms GraphPad Prism 9.0 GraphPad Software N/A R v4.3.0 R N/A Zen Pro Zeiss N/A Quantity One 1-D Analysis Software Bio-Rad N/A Python Python https://www.python.org/ Zeiss Zen Pro Software Zeiss N/A Standard inverted multichannel Carl Zeiss Axio Obeserver Micropscope (Leica camera 20x objective) Zeiss N/A Fiji https://fiji.sc QuPath Image Processing Software QuPath https://qupath.github.io/ QuantStudio 7 Real-Time PCR Thermo Fisher Scientific N/A BioNavigator PamGene N/A Cytoscape Cytoscape Team https://cytoscape.org/ NIS Elements Software Nikon N/A Nikon inverted TiE microscope Nikon N/A Article ll OPEN ACCESS ..

    Multiplex Assay:

    Article Title: Exercise-induced crosstalk between immune cells and adipocytes in humans: Role of oncostatin-M
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) 1:1000 Cell Signaling Technology #9101; RRID:AB_331646 Phospho-HSL (Ser563) 1:1000 Cell Signaling Technology #4139; RRID:AB_2135495 Phospho-Stat3 (Tyr705) (D3A7) XP 1:1000 Cell Signaling Technology #9145; RRID:AB_2491009 Itgam 1:500 Atlas Antibodies AMAb90911; RRID:AB_2665722 Biological samples Primary human myoblasts Previous works from the group N/A Human in vitro differentiated adipocytes Gao et al., 201759 N/A Human subcutaneous leg fat biopsies This paper N/A Human plasma samples This paper N/A Human subcutaneous abdominal fat biopsies Fabre et al.20 N/A Chemicals, peptides, and recombinant proteins Oncostatin-M Bio-Techne LTD 295-OM-050 GDF15 Bio-Techne LTD 9279-GD-050 SFRP4 Bio-Techne LTD 1827-SF-025/CF MRXA5 Nordic Biosite LS-G26239-10 Isoproterenol Merck I5627 Clenbuterol Merck C5423-10MG Forskolin Merck F6886-10MG IBMX Merck I5879-250MG Insulin Novo Nordisk Actrapid Dexamethasone Merck D4902-1G Rosiglitazone GlaxoSmithKline BRL49653-C apo-transferrin BBI solution T100-5 Recombinant Human IL4 PeProTech 200–04 Recombinant Human IL13 PeProTech 200–13 Recombinant Human IFNg PeProTech 300–02 Phorbol 12-myristate 13-acetate (PMA) Merck/Sigma-Aldrich P1585 Lipopolysaccharides Merck/Sigma-Aldrich L9641 Hoechst 33342 Abcam Ab228551 Lectin Vector Laboratories DL-1048-1 TRIzol Thermo Fisher Scientific 15596018 Triton X-100 Sigma-Aldrich X100-100ML Tween 20 Sigma-Aldrich P1379-1L DAKO Fluorescence mounting media Agilent Technologies S302380-2 Protein Block Agilent Technologies X090930-2 Protease Inhibitor Cocktail CALBIOCHEM 539131–10 ECL reagent GE Healthcare RPN2106 Critical commercial assays TruSeq Stranded Total RNA with Ribo-Zero Gold Illumina 20020598 Rneasy lipid tissue kit Qiagen Cat#74804 Agilent RNA 6000 nano kit Agilent 5067–1511 Qubit dsDNA HS assay kit Invitrogen Q32851 Agilent High Sensitivity DNA chip Agilent 5067–4626 Human Oncostatin M/OSM ELISA Kit Abcam ab215543 (Continued on next page) e1 Cell Reports Medicine 5, 101348, January 16, 2024 .. REAGENT or RESOURCE SOURCE IDENTIFIER EZNA total RNA kit I Omega Bio-tek R6834-01 Pierce BCA Protein Assay Kit ThermoFisher Cat#23227 Multiplex Fluorescent detection reagents v2 ACDBio 323110 Free glycerol Reagent Sigma-Aldrich #F6428 Amplex Ultrared Reagent Invitrogen #A36006 Deposited data Human adipose tissue in response to exercise (RNAseq) This paper GEO: GSE198922 Experimental models: Cell lines Thp1 monocytes cell line ATCC TIB-202 Oligonucleotides TaqMan probe human OSM Applied Biosystems Hs00171165_m1 TaqMan probe human GDF15 Applied Biosystems Hs00171132_m1 TaqMan probe human LIF Applied Biosystems Hs01055668_m1 TaqMan probe human OSMR Applied Biosystems Hs00384276_m1 TaqMan probe human LIFR Applied Biosystems Hs01123581_m1 TaqMan probe human IL6ST Applied Biosystems Hs00174360_m1 TaqMan probe human IL6 Applied Biosystems Hs00174131_m1 TaqMan probe human CXCL2 Applied Biosystems Hs00601975_m1 RNAscopeTM Probe- Hs-OSM ACDBio 456381 RNAscopeTM Probe- Hs-CD68-C2 ACDBio 560591-C2 Software and algorithms GraphPad Prism 9.0 GraphPad Software N/A R v4.3.0 R N/A Zen Pro Zeiss N/A Quantity One 1-D Analysis Software Bio-Rad N/A Python Python https://www.python.org/ Zeiss Zen Pro Software Zeiss N/A Standard inverted multichannel Carl Zeiss Axio Obeserver Micropscope (Leica camera 20x objective) Zeiss N/A Fiji https://fiji.sc QuPath Image Processing Software QuPath https://qupath.github.io/ QuantStudio 7 Real-Time PCR Thermo Fisher Scientific N/A BioNavigator PamGene N/A Cytoscape Cytoscape Team https://cytoscape.org/ NIS Elements Software Nikon N/A Nikon inverted TiE microscope Nikon N/A Article ll OPEN ACCESS ..

    RNA sequencing:

    Article Title: Exercise-induced crosstalk between immune cells and adipocytes in humans: Role of oncostatin-M
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) 1:1000 Cell Signaling Technology #9101; RRID:AB_331646 Phospho-HSL (Ser563) 1:1000 Cell Signaling Technology #4139; RRID:AB_2135495 Phospho-Stat3 (Tyr705) (D3A7) XP 1:1000 Cell Signaling Technology #9145; RRID:AB_2491009 Itgam 1:500 Atlas Antibodies AMAb90911; RRID:AB_2665722 Biological samples Primary human myoblasts Previous works from the group N/A Human in vitro differentiated adipocytes Gao et al., 201759 N/A Human subcutaneous leg fat biopsies This paper N/A Human plasma samples This paper N/A Human subcutaneous abdominal fat biopsies Fabre et al.20 N/A Chemicals, peptides, and recombinant proteins Oncostatin-M Bio-Techne LTD 295-OM-050 GDF15 Bio-Techne LTD 9279-GD-050 SFRP4 Bio-Techne LTD 1827-SF-025/CF MRXA5 Nordic Biosite LS-G26239-10 Isoproterenol Merck I5627 Clenbuterol Merck C5423-10MG Forskolin Merck F6886-10MG IBMX Merck I5879-250MG Insulin Novo Nordisk Actrapid Dexamethasone Merck D4902-1G Rosiglitazone GlaxoSmithKline BRL49653-C apo-transferrin BBI solution T100-5 Recombinant Human IL4 PeProTech 200–04 Recombinant Human IL13 PeProTech 200–13 Recombinant Human IFNg PeProTech 300–02 Phorbol 12-myristate 13-acetate (PMA) Merck/Sigma-Aldrich P1585 Lipopolysaccharides Merck/Sigma-Aldrich L9641 Hoechst 33342 Abcam Ab228551 Lectin Vector Laboratories DL-1048-1 TRIzol Thermo Fisher Scientific 15596018 Triton X-100 Sigma-Aldrich X100-100ML Tween 20 Sigma-Aldrich P1379-1L DAKO Fluorescence mounting media Agilent Technologies S302380-2 Protein Block Agilent Technologies X090930-2 Protease Inhibitor Cocktail CALBIOCHEM 539131–10 ECL reagent GE Healthcare RPN2106 Critical commercial assays TruSeq Stranded Total RNA with Ribo-Zero Gold Illumina 20020598 Rneasy lipid tissue kit Qiagen Cat#74804 Agilent RNA 6000 nano kit Agilent 5067–1511 Qubit dsDNA HS assay kit Invitrogen Q32851 Agilent High Sensitivity DNA chip Agilent 5067–4626 Human Oncostatin M/OSM ELISA Kit Abcam ab215543 (Continued on next page) e1 Cell Reports Medicine 5, 101348, January 16, 2024 .. REAGENT or RESOURCE SOURCE IDENTIFIER EZNA total RNA kit I Omega Bio-tek R6834-01 Pierce BCA Protein Assay Kit ThermoFisher Cat#23227 Multiplex Fluorescent detection reagents v2 ACDBio 323110 Free glycerol Reagent Sigma-Aldrich #F6428 Amplex Ultrared Reagent Invitrogen #A36006 Deposited data Human adipose tissue in response to exercise (RNAseq) This paper GEO: GSE198922 Experimental models: Cell lines Thp1 monocytes cell line ATCC TIB-202 Oligonucleotides TaqMan probe human OSM Applied Biosystems Hs00171165_m1 TaqMan probe human GDF15 Applied Biosystems Hs00171132_m1 TaqMan probe human LIF Applied Biosystems Hs01055668_m1 TaqMan probe human OSMR Applied Biosystems Hs00384276_m1 TaqMan probe human LIFR Applied Biosystems Hs01123581_m1 TaqMan probe human IL6ST Applied Biosystems Hs00174360_m1 TaqMan probe human IL6 Applied Biosystems Hs00174131_m1 TaqMan probe human CXCL2 Applied Biosystems Hs00601975_m1 RNAscopeTM Probe- Hs-OSM ACDBio 456381 RNAscopeTM Probe- Hs-CD68-C2 ACDBio 560591-C2 Software and algorithms GraphPad Prism 9.0 GraphPad Software N/A R v4.3.0 R N/A Zen Pro Zeiss N/A Quantity One 1-D Analysis Software Bio-Rad N/A Python Python https://www.python.org/ Zeiss Zen Pro Software Zeiss N/A Standard inverted multichannel Carl Zeiss Axio Obeserver Micropscope (Leica camera 20x objective) Zeiss N/A Fiji https://fiji.sc QuPath Image Processing Software QuPath https://qupath.github.io/ QuantStudio 7 Real-Time PCR Thermo Fisher Scientific N/A BioNavigator PamGene N/A Cytoscape Cytoscape Team https://cytoscape.org/ NIS Elements Software Nikon N/A Nikon inverted TiE microscope Nikon N/A Article ll OPEN ACCESS ..

    Software:

    Article Title: Exercise-induced crosstalk between immune cells and adipocytes in humans: Role of oncostatin-M
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) 1:1000 Cell Signaling Technology #9101; RRID:AB_331646 Phospho-HSL (Ser563) 1:1000 Cell Signaling Technology #4139; RRID:AB_2135495 Phospho-Stat3 (Tyr705) (D3A7) XP 1:1000 Cell Signaling Technology #9145; RRID:AB_2491009 Itgam 1:500 Atlas Antibodies AMAb90911; RRID:AB_2665722 Biological samples Primary human myoblasts Previous works from the group N/A Human in vitro differentiated adipocytes Gao et al., 201759 N/A Human subcutaneous leg fat biopsies This paper N/A Human plasma samples This paper N/A Human subcutaneous abdominal fat biopsies Fabre et al.20 N/A Chemicals, peptides, and recombinant proteins Oncostatin-M Bio-Techne LTD 295-OM-050 GDF15 Bio-Techne LTD 9279-GD-050 SFRP4 Bio-Techne LTD 1827-SF-025/CF MRXA5 Nordic Biosite LS-G26239-10 Isoproterenol Merck I5627 Clenbuterol Merck C5423-10MG Forskolin Merck F6886-10MG IBMX Merck I5879-250MG Insulin Novo Nordisk Actrapid Dexamethasone Merck D4902-1G Rosiglitazone GlaxoSmithKline BRL49653-C apo-transferrin BBI solution T100-5 Recombinant Human IL4 PeProTech 200–04 Recombinant Human IL13 PeProTech 200–13 Recombinant Human IFNg PeProTech 300–02 Phorbol 12-myristate 13-acetate (PMA) Merck/Sigma-Aldrich P1585 Lipopolysaccharides Merck/Sigma-Aldrich L9641 Hoechst 33342 Abcam Ab228551 Lectin Vector Laboratories DL-1048-1 TRIzol Thermo Fisher Scientific 15596018 Triton X-100 Sigma-Aldrich X100-100ML Tween 20 Sigma-Aldrich P1379-1L DAKO Fluorescence mounting media Agilent Technologies S302380-2 Protein Block Agilent Technologies X090930-2 Protease Inhibitor Cocktail CALBIOCHEM 539131–10 ECL reagent GE Healthcare RPN2106 Critical commercial assays TruSeq Stranded Total RNA with Ribo-Zero Gold Illumina 20020598 Rneasy lipid tissue kit Qiagen Cat#74804 Agilent RNA 6000 nano kit Agilent 5067–1511 Qubit dsDNA HS assay kit Invitrogen Q32851 Agilent High Sensitivity DNA chip Agilent 5067–4626 Human Oncostatin M/OSM ELISA Kit Abcam ab215543 (Continued on next page) e1 Cell Reports Medicine 5, 101348, January 16, 2024 .. REAGENT or RESOURCE SOURCE IDENTIFIER EZNA total RNA kit I Omega Bio-tek R6834-01 Pierce BCA Protein Assay Kit ThermoFisher Cat#23227 Multiplex Fluorescent detection reagents v2 ACDBio 323110 Free glycerol Reagent Sigma-Aldrich #F6428 Amplex Ultrared Reagent Invitrogen #A36006 Deposited data Human adipose tissue in response to exercise (RNAseq) This paper GEO: GSE198922 Experimental models: Cell lines Thp1 monocytes cell line ATCC TIB-202 Oligonucleotides TaqMan probe human OSM Applied Biosystems Hs00171165_m1 TaqMan probe human GDF15 Applied Biosystems Hs00171132_m1 TaqMan probe human LIF Applied Biosystems Hs01055668_m1 TaqMan probe human OSMR Applied Biosystems Hs00384276_m1 TaqMan probe human LIFR Applied Biosystems Hs01123581_m1 TaqMan probe human IL6ST Applied Biosystems Hs00174360_m1 TaqMan probe human IL6 Applied Biosystems Hs00174131_m1 TaqMan probe human CXCL2 Applied Biosystems Hs00601975_m1 RNAscopeTM Probe- Hs-OSM ACDBio 456381 RNAscopeTM Probe- Hs-CD68-C2 ACDBio 560591-C2 Software and algorithms GraphPad Prism 9.0 GraphPad Software N/A R v4.3.0 R N/A Zen Pro Zeiss N/A Quantity One 1-D Analysis Software Bio-Rad N/A Python Python https://www.python.org/ Zeiss Zen Pro Software Zeiss N/A Standard inverted multichannel Carl Zeiss Axio Obeserver Micropscope (Leica camera 20x objective) Zeiss N/A Fiji https://fiji.sc QuPath Image Processing Software QuPath https://qupath.github.io/ QuantStudio 7 Real-Time PCR Thermo Fisher Scientific N/A BioNavigator PamGene N/A Cytoscape Cytoscape Team https://cytoscape.org/ NIS Elements Software Nikon N/A Nikon inverted TiE microscope Nikon N/A Article ll OPEN ACCESS ..

    Article Title: Cordycepin-based derivatized compound with anti-tumor effect
    Article Snippet: .. After the zebrafish in each experimental group were continuously cultured at 35° C. to be 5 dpf, 10 zebrafish in each experimental group were randomly selected, observed under a fluorescence microscope, and photographed, images were saved, and analyzed by Nikon NIS-Elements l3.10 advanced image processing software, and fluorescence intensities (S) of transplanted tumors of the zebrafish were calculated. ..

    Article Title: Implications of holdfast availability and patchiness on juvenile seahorse ( <i>Hippocampus reidi</i> ) condition and welfare: Simulations in captivity
    Article Snippet: Treatments:W – without ropes; M –medium density ropes; H – high-density ropes to Petri dishes, photographed and weighed individually on a Sartorius microbalance MC210P (± 0.01 mg). .. Curved standard lengths (SL) were measured (SL = head + trunk + curved tail) from digital photographs using an image processing software (NIS Elements Microscopy Software, Nikon). ..

    Article Title: Identifying Baicalein as a Key Bioactive Compound in XueBiJing Targeting KEAP1: Implications for Antioxidant Effects
    Article Snippet: .. After being treated for 3 h at 28 °C, 10 zebrafish from each group were randomly selected to be observed, and optical images were captured under a fluorescent microscope (Nikon AZ100, Minato City, Japan) and then analyzed by image processing software (NIS-Elements D 3.20). ..

    Article Title: The analysis of X chromosome activity of porcine embryonic stem Cells: Study based on parthenogenetic embryonic stem cells with LCDM medium.
    Article Snippet: The derivation of porcine embryonic stem cell (pESC) lines remains a major challenge in this field.. To date, the porcine naïve ESCs have yet to be successfully established, and standardized criteria for their characterization and evaluation are still lacking.. The regulation of X-chromosome activity integrates information from embryonic development and the dosage of sex chromosomes, which is closely associated with the pluripotent state of embryonic stem cells.

    Article Title: Microbial and chemical transformations of euphorbia factor L1 and the P-glycoprotein inhibitory activity in zebrafishes.
    Article Snippet: .. Zebrafish images were recorded by a Nikon AZ100 multizoom microscope, and the quantitative image analysis was conducted by measuring FI with Nikon NIS-Elements D.310 advanced image processing software (Tokyo, Japan). ..

    Article Title: Identifying Baicalein as a Key Bioactive Compound in XueBiJing Targeting KEAP1: Implications for Antioxidant Effects
    Article Snippet: .. Except for the normal control group, all other experimental groups were given Poly (I:C) (100 ng/fish) by swim bladder injection to establish a zebrafish viral inflammation model. After being treated for 3 h at 28 ◦C, 10 zebrafish from each group were randomly selected to be observed, and optical images were captured under a fluorescent microscope (Nikon AZ100, Minato City, Japan) and then analyzed by image processing software (NIS-Elements D 3.20). ..

    Real-time Polymerase Chain Reaction:

    Article Title: Exercise-induced crosstalk between immune cells and adipocytes in humans: Role of oncostatin-M
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) 1:1000 Cell Signaling Technology #9101; RRID:AB_331646 Phospho-HSL (Ser563) 1:1000 Cell Signaling Technology #4139; RRID:AB_2135495 Phospho-Stat3 (Tyr705) (D3A7) XP 1:1000 Cell Signaling Technology #9145; RRID:AB_2491009 Itgam 1:500 Atlas Antibodies AMAb90911; RRID:AB_2665722 Biological samples Primary human myoblasts Previous works from the group N/A Human in vitro differentiated adipocytes Gao et al., 201759 N/A Human subcutaneous leg fat biopsies This paper N/A Human plasma samples This paper N/A Human subcutaneous abdominal fat biopsies Fabre et al.20 N/A Chemicals, peptides, and recombinant proteins Oncostatin-M Bio-Techne LTD 295-OM-050 GDF15 Bio-Techne LTD 9279-GD-050 SFRP4 Bio-Techne LTD 1827-SF-025/CF MRXA5 Nordic Biosite LS-G26239-10 Isoproterenol Merck I5627 Clenbuterol Merck C5423-10MG Forskolin Merck F6886-10MG IBMX Merck I5879-250MG Insulin Novo Nordisk Actrapid Dexamethasone Merck D4902-1G Rosiglitazone GlaxoSmithKline BRL49653-C apo-transferrin BBI solution T100-5 Recombinant Human IL4 PeProTech 200–04 Recombinant Human IL13 PeProTech 200–13 Recombinant Human IFNg PeProTech 300–02 Phorbol 12-myristate 13-acetate (PMA) Merck/Sigma-Aldrich P1585 Lipopolysaccharides Merck/Sigma-Aldrich L9641 Hoechst 33342 Abcam Ab228551 Lectin Vector Laboratories DL-1048-1 TRIzol Thermo Fisher Scientific 15596018 Triton X-100 Sigma-Aldrich X100-100ML Tween 20 Sigma-Aldrich P1379-1L DAKO Fluorescence mounting media Agilent Technologies S302380-2 Protein Block Agilent Technologies X090930-2 Protease Inhibitor Cocktail CALBIOCHEM 539131–10 ECL reagent GE Healthcare RPN2106 Critical commercial assays TruSeq Stranded Total RNA with Ribo-Zero Gold Illumina 20020598 Rneasy lipid tissue kit Qiagen Cat#74804 Agilent RNA 6000 nano kit Agilent 5067–1511 Qubit dsDNA HS assay kit Invitrogen Q32851 Agilent High Sensitivity DNA chip Agilent 5067–4626 Human Oncostatin M/OSM ELISA Kit Abcam ab215543 (Continued on next page) e1 Cell Reports Medicine 5, 101348, January 16, 2024 .. REAGENT or RESOURCE SOURCE IDENTIFIER EZNA total RNA kit I Omega Bio-tek R6834-01 Pierce BCA Protein Assay Kit ThermoFisher Cat#23227 Multiplex Fluorescent detection reagents v2 ACDBio 323110 Free glycerol Reagent Sigma-Aldrich #F6428 Amplex Ultrared Reagent Invitrogen #A36006 Deposited data Human adipose tissue in response to exercise (RNAseq) This paper GEO: GSE198922 Experimental models: Cell lines Thp1 monocytes cell line ATCC TIB-202 Oligonucleotides TaqMan probe human OSM Applied Biosystems Hs00171165_m1 TaqMan probe human GDF15 Applied Biosystems Hs00171132_m1 TaqMan probe human LIF Applied Biosystems Hs01055668_m1 TaqMan probe human OSMR Applied Biosystems Hs00384276_m1 TaqMan probe human LIFR Applied Biosystems Hs01123581_m1 TaqMan probe human IL6ST Applied Biosystems Hs00174360_m1 TaqMan probe human IL6 Applied Biosystems Hs00174131_m1 TaqMan probe human CXCL2 Applied Biosystems Hs00601975_m1 RNAscopeTM Probe- Hs-OSM ACDBio 456381 RNAscopeTM Probe- Hs-CD68-C2 ACDBio 560591-C2 Software and algorithms GraphPad Prism 9.0 GraphPad Software N/A R v4.3.0 R N/A Zen Pro Zeiss N/A Quantity One 1-D Analysis Software Bio-Rad N/A Python Python https://www.python.org/ Zeiss Zen Pro Software Zeiss N/A Standard inverted multichannel Carl Zeiss Axio Obeserver Micropscope (Leica camera 20x objective) Zeiss N/A Fiji https://fiji.sc QuPath Image Processing Software QuPath https://qupath.github.io/ QuantStudio 7 Real-Time PCR Thermo Fisher Scientific N/A BioNavigator PamGene N/A Cytoscape Cytoscape Team https://cytoscape.org/ NIS Elements Software Nikon N/A Nikon inverted TiE microscope Nikon N/A Article ll OPEN ACCESS ..

    Microscopy:

    Article Title: Exercise-induced crosstalk between immune cells and adipocytes in humans: Role of oncostatin-M
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) 1:1000 Cell Signaling Technology #9101; RRID:AB_331646 Phospho-HSL (Ser563) 1:1000 Cell Signaling Technology #4139; RRID:AB_2135495 Phospho-Stat3 (Tyr705) (D3A7) XP 1:1000 Cell Signaling Technology #9145; RRID:AB_2491009 Itgam 1:500 Atlas Antibodies AMAb90911; RRID:AB_2665722 Biological samples Primary human myoblasts Previous works from the group N/A Human in vitro differentiated adipocytes Gao et al., 201759 N/A Human subcutaneous leg fat biopsies This paper N/A Human plasma samples This paper N/A Human subcutaneous abdominal fat biopsies Fabre et al.20 N/A Chemicals, peptides, and recombinant proteins Oncostatin-M Bio-Techne LTD 295-OM-050 GDF15 Bio-Techne LTD 9279-GD-050 SFRP4 Bio-Techne LTD 1827-SF-025/CF MRXA5 Nordic Biosite LS-G26239-10 Isoproterenol Merck I5627 Clenbuterol Merck C5423-10MG Forskolin Merck F6886-10MG IBMX Merck I5879-250MG Insulin Novo Nordisk Actrapid Dexamethasone Merck D4902-1G Rosiglitazone GlaxoSmithKline BRL49653-C apo-transferrin BBI solution T100-5 Recombinant Human IL4 PeProTech 200–04 Recombinant Human IL13 PeProTech 200–13 Recombinant Human IFNg PeProTech 300–02 Phorbol 12-myristate 13-acetate (PMA) Merck/Sigma-Aldrich P1585 Lipopolysaccharides Merck/Sigma-Aldrich L9641 Hoechst 33342 Abcam Ab228551 Lectin Vector Laboratories DL-1048-1 TRIzol Thermo Fisher Scientific 15596018 Triton X-100 Sigma-Aldrich X100-100ML Tween 20 Sigma-Aldrich P1379-1L DAKO Fluorescence mounting media Agilent Technologies S302380-2 Protein Block Agilent Technologies X090930-2 Protease Inhibitor Cocktail CALBIOCHEM 539131–10 ECL reagent GE Healthcare RPN2106 Critical commercial assays TruSeq Stranded Total RNA with Ribo-Zero Gold Illumina 20020598 Rneasy lipid tissue kit Qiagen Cat#74804 Agilent RNA 6000 nano kit Agilent 5067–1511 Qubit dsDNA HS assay kit Invitrogen Q32851 Agilent High Sensitivity DNA chip Agilent 5067–4626 Human Oncostatin M/OSM ELISA Kit Abcam ab215543 (Continued on next page) e1 Cell Reports Medicine 5, 101348, January 16, 2024 .. REAGENT or RESOURCE SOURCE IDENTIFIER EZNA total RNA kit I Omega Bio-tek R6834-01 Pierce BCA Protein Assay Kit ThermoFisher Cat#23227 Multiplex Fluorescent detection reagents v2 ACDBio 323110 Free glycerol Reagent Sigma-Aldrich #F6428 Amplex Ultrared Reagent Invitrogen #A36006 Deposited data Human adipose tissue in response to exercise (RNAseq) This paper GEO: GSE198922 Experimental models: Cell lines Thp1 monocytes cell line ATCC TIB-202 Oligonucleotides TaqMan probe human OSM Applied Biosystems Hs00171165_m1 TaqMan probe human GDF15 Applied Biosystems Hs00171132_m1 TaqMan probe human LIF Applied Biosystems Hs01055668_m1 TaqMan probe human OSMR Applied Biosystems Hs00384276_m1 TaqMan probe human LIFR Applied Biosystems Hs01123581_m1 TaqMan probe human IL6ST Applied Biosystems Hs00174360_m1 TaqMan probe human IL6 Applied Biosystems Hs00174131_m1 TaqMan probe human CXCL2 Applied Biosystems Hs00601975_m1 RNAscopeTM Probe- Hs-OSM ACDBio 456381 RNAscopeTM Probe- Hs-CD68-C2 ACDBio 560591-C2 Software and algorithms GraphPad Prism 9.0 GraphPad Software N/A R v4.3.0 R N/A Zen Pro Zeiss N/A Quantity One 1-D Analysis Software Bio-Rad N/A Python Python https://www.python.org/ Zeiss Zen Pro Software Zeiss N/A Standard inverted multichannel Carl Zeiss Axio Obeserver Micropscope (Leica camera 20x objective) Zeiss N/A Fiji https://fiji.sc QuPath Image Processing Software QuPath https://qupath.github.io/ QuantStudio 7 Real-Time PCR Thermo Fisher Scientific N/A BioNavigator PamGene N/A Cytoscape Cytoscape Team https://cytoscape.org/ NIS Elements Software Nikon N/A Nikon inverted TiE microscope Nikon N/A Article ll OPEN ACCESS ..

    Article Title: Cordycepin-based derivatized compound with anti-tumor effect
    Article Snippet: .. After the zebrafish in each experimental group were continuously cultured at 35° C. to be 5 dpf, 10 zebrafish in each experimental group were randomly selected, observed under a fluorescence microscope, and photographed, images were saved, and analyzed by Nikon NIS-Elements l3.10 advanced image processing software, and fluorescence intensities (S) of transplanted tumors of the zebrafish were calculated. ..

    Article Title: Implications of holdfast availability and patchiness on juvenile seahorse ( <i>Hippocampus reidi</i> ) condition and welfare: Simulations in captivity
    Article Snippet: Treatments:W – without ropes; M –medium density ropes; H – high-density ropes to Petri dishes, photographed and weighed individually on a Sartorius microbalance MC210P (± 0.01 mg). .. Curved standard lengths (SL) were measured (SL = head + trunk + curved tail) from digital photographs using an image processing software (NIS Elements Microscopy Software, Nikon). ..

    Article Title: Identifying Baicalein as a Key Bioactive Compound in XueBiJing Targeting KEAP1: Implications for Antioxidant Effects
    Article Snippet: .. After being treated for 3 h at 28 °C, 10 zebrafish from each group were randomly selected to be observed, and optical images were captured under a fluorescent microscope (Nikon AZ100, Minato City, Japan) and then analyzed by image processing software (NIS-Elements D 3.20). ..

    Article Title: The analysis of X chromosome activity of porcine embryonic stem Cells: Study based on parthenogenetic embryonic stem cells with LCDM medium.
    Article Snippet: The derivation of porcine embryonic stem cell (pESC) lines remains a major challenge in this field.. To date, the porcine naïve ESCs have yet to be successfully established, and standardized criteria for their characterization and evaluation are still lacking.. The regulation of X-chromosome activity integrates information from embryonic development and the dosage of sex chromosomes, which is closely associated with the pluripotent state of embryonic stem cells.

    Article Title: Microbial and chemical transformations of euphorbia factor L1 and the P-glycoprotein inhibitory activity in zebrafishes.
    Article Snippet: .. Zebrafish images were recorded by a Nikon AZ100 multizoom microscope, and the quantitative image analysis was conducted by measuring FI with Nikon NIS-Elements D.310 advanced image processing software (Tokyo, Japan). ..

    Article Title: Identifying Baicalein as a Key Bioactive Compound in XueBiJing Targeting KEAP1: Implications for Antioxidant Effects
    Article Snippet: .. Except for the normal control group, all other experimental groups were given Poly (I:C) (100 ng/fish) by swim bladder injection to establish a zebrafish viral inflammation model. After being treated for 3 h at 28 ◦C, 10 zebrafish from each group were randomly selected to be observed, and optical images were captured under a fluorescent microscope (Nikon AZ100, Minato City, Japan) and then analyzed by image processing software (NIS-Elements D 3.20). ..

    Cell Culture:

    Article Title: Cordycepin-based derivatized compound with anti-tumor effect
    Article Snippet: .. After the zebrafish in each experimental group were continuously cultured at 35° C. to be 5 dpf, 10 zebrafish in each experimental group were randomly selected, observed under a fluorescence microscope, and photographed, images were saved, and analyzed by Nikon NIS-Elements l3.10 advanced image processing software, and fluorescence intensities (S) of transplanted tumors of the zebrafish were calculated. ..

    Fluorescence:

    Article Title: Cordycepin-based derivatized compound with anti-tumor effect
    Article Snippet: .. After the zebrafish in each experimental group were continuously cultured at 35° C. to be 5 dpf, 10 zebrafish in each experimental group were randomly selected, observed under a fluorescence microscope, and photographed, images were saved, and analyzed by Nikon NIS-Elements l3.10 advanced image processing software, and fluorescence intensities (S) of transplanted tumors of the zebrafish were calculated. ..

    Article Title: The analysis of X chromosome activity of porcine embryonic stem Cells: Study based on parthenogenetic embryonic stem cells with LCDM medium.
    Article Snippet: The derivation of porcine embryonic stem cell (pESC) lines remains a major challenge in this field.. To date, the porcine naïve ESCs have yet to be successfully established, and standardized criteria for their characterization and evaluation are still lacking.. The regulation of X-chromosome activity integrates information from embryonic development and the dosage of sex chromosomes, which is closely associated with the pluripotent state of embryonic stem cells.

    Incubation:

    Article Title: The analysis of X chromosome activity of porcine embryonic stem Cells: Study based on parthenogenetic embryonic stem cells with LCDM medium.
    Article Snippet: The derivation of porcine embryonic stem cell (pESC) lines remains a major challenge in this field.. To date, the porcine naïve ESCs have yet to be successfully established, and standardized criteria for their characterization and evaluation are still lacking.. The regulation of X-chromosome activity integrates information from embryonic development and the dosage of sex chromosomes, which is closely associated with the pluripotent state of embryonic stem cells.

    Control:

    Article Title: Identifying Baicalein as a Key Bioactive Compound in XueBiJing Targeting KEAP1: Implications for Antioxidant Effects
    Article Snippet: .. Except for the normal control group, all other experimental groups were given Poly (I:C) (100 ng/fish) by swim bladder injection to establish a zebrafish viral inflammation model. After being treated for 3 h at 28 ◦C, 10 zebrafish from each group were randomly selected to be observed, and optical images were captured under a fluorescent microscope (Nikon AZ100, Minato City, Japan) and then analyzed by image processing software (NIS-Elements D 3.20). ..

    Injection:

    Article Title: Identifying Baicalein as a Key Bioactive Compound in XueBiJing Targeting KEAP1: Implications for Antioxidant Effects
    Article Snippet: .. Except for the normal control group, all other experimental groups were given Poly (I:C) (100 ng/fish) by swim bladder injection to establish a zebrafish viral inflammation model. After being treated for 3 h at 28 ◦C, 10 zebrafish from each group were randomly selected to be observed, and optical images were captured under a fluorescent microscope (Nikon AZ100, Minato City, Japan) and then analyzed by image processing software (NIS-Elements D 3.20). ..



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